Background Epicutaneous immunotherapy (EPIT) on undamaged skin with an epicutaneous delivery system was already found in preclinical and medical studies. Outcomes EPIT on undamaged pores and skin significantly decreased Th2 immunological response (IgE response and splenocyte secretion of Th2 cytokines) aswell as esophageal eosinophilia (2.7??0.9, in comparison to Sham 19.9??1.5, p?0.01), mRNA manifestation of Th2 cytokines in cells and intestinal villus sub-atrophia (2.9??0.2 vs Sham, 2.1??0.2, p?0.05). In comparison, EPIT on stripped pores and skin strengthened Th2 systemic immunological response aswell as eosinophil infiltration (26.8??15.1), mRNA manifestation of Th2 cytokines and duodenal villus/crypt-ratio (2.4??0.3). Conclusions Dinaciclib Epicutaneous allergen-specific immunotherapy requirements the integrity of superficial levels from the stratum corneum to guarantee protection of treatment also to stimulate a tolerogenic profile from the immune system response. test. Outcomes Protection of epicutaneous software on undamaged pores and skin instead of stripped pores and skin In mice treated by subcutaneous shot of 500g of PPE, offering as positive settings of delivery in to the bloodstream, a higher level of Ara h 1 was recognized from 2h to 48h, having a peak at 8h (147.5??20.6ng/ml) (Physique ?(Figure2).2). When Viaskin?-500 was applied on intact skin, no Ara h 1 was detected in the serum from 0 to 48h. When Viaskin?-500 was applied on stripped skin, a limited quantity of Ara h 1 was detected in the serum at 2h (39.5??21.2ng/ml) and 8h (10.8??5.4ng/ml) after the application. For both EPIT groups, the quantity of PPE remaining inside the Viaskin? after 48h was measured at a similar level (25g for EPIT and 20g for stripping+EPIT, quantified by total protein assay) whereas the quantity transferred into the skin (epidermis and dermis) was a little higher at 2h and 8h for the intact skin Dinaciclib group (data not shown, 1007ng/ml and 388ng/ml for EPIT vs 677ng/ml and 146ng/ml for stripping+EPIT). Physique 2 Quantification of Ara h 1 in serum sample of mice. Quantity of Ara h 1 was measured in serum samples after epicutaneous administration on intact or stripped skin or subcutaneous administration of 500g of PPE. Results were expressed in ng/ml as … Dinaciclib Modulation of humoral/cellular responses by EPIT depending on the integrity of epidermis The serological responses were analyzed after both sensitization (D42) and a 8-week EPIT (D106) (Physique ?(Figure3).3). No specific antibodies to PPE were detected for naive mice. In the sham group, specific IgE increased significantly after sensitization and were maintained during 8-week of treatment, with no modification of specific IgG2a. When EPIT was applied on intact skin, specific IgE decreased from D42 to D106 (from 0.14 to 0.04 g/ml, p<0.05) and specific IgG2a increased (from 0.56 to 3.21 g/ml, p<0.05). To the opposite, when EPIT was applied on stripped skin, specific IgE increased (from 0.12 to 0.38 g/ml, p<0.01) and specific IgG2a were not modified (0.98 vs 1.25 g/ml, ns). The IgG1/IgG2a ratio significantly differed between EPIT and Sham or stripping+EPIT (respectively, 18 vs 228 or 227, p<0.001). Physique 3 Systemic responses induced in mice after oral sensitization and epicutaneous immunotherapy (a) Quantity of specific IgE and (b) specific IgG2a expressed in g/ml. Data are expressed as means SD for each group, D42 after oral sensitization, ... Levels of histamine released in plasma sampled 30 min after oral challenge were higher in sham (1384 nM) than in naive mice (317 nM, p<0.001). It was significantly reduced by EPIT done in intact skin (369 nM, p<0.01 vs. sham) HERPUD1 whereas the release was still high for mice treated by EPIT applied on stripped skin (1028 nM, p<0.01 vs naive and EPIT). Splenocytes were reactivated in vitro in presence of PPE. In sham mice, they specifically secreted Th1 and mainly Th2 cytokines in comparison to naive mice (Physique ?(Figure4):4): IL-4 (46.5 vs 2.4 pg/ml, p<0.01), IL-5 (148.3 vs 11.0, p<0.01), Dinaciclib IL-13 (154.6 vs 7.3, p<0.01) and IFN- (75.9 vs 3.9, p<0.01). When mice were treated by EPIT on intact skin, Th2.